Applications:

We provide comprehensive training on all microscopes and can assist in experimental design, implementation and analysis.

Reserve a microscope

Fixed tissue confocal microscopy including:

  • Multi-fluorophore staining immunofluorescence
  • Imaging through thick tissues
  • Spectral deconvolution META Analysis (up to 8 fluorophores)
  • Removal of autofluorescence
  • Quantum dot imaging
  • FRET, FRAP
  • These applications use the Zeiss LSM 510 META

Live cell confocal imaging, including:

  • Dynamic intra-cellular events
  • Intracellular trafficking
  • Quantum dot imaging and uptake
  • Long and short term drug treatments
  • Cell division
  • Cell migration
  • Can be combined with microinjection
  • These applications use the Perkin Elmer Ultraview ERS
  • Movies using this microscope

Immunohistochemistry and fluorescence microscopy

  • Color camera imaging of IHC stained tissue sections
  • Fluorescence imaging
  • User friendly image capture
  • These applications use the Zeiss Axioplan 2

Microinjection

  • Automated microinjection of plasmids, siRNA, quantum dots, dyes, stains, etc.
  • Attached to live cell confocal imaging microscope
  • These applications use the Eppendorf Microinjector

Image analysis, including:

  • 3-D Deconvolution
  • Quantitative analyses (intensity, morphology, shape, counts, etc.)
  • Colocalization
  • FRET and FRAP analysis
  • Densitometry
  • Object tracking
  • 3-D rotations
  • These applications use Metamorph and AutoDeblur